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Sunday, August 9, 2020 | History

2 edition of Recognition of "extended-10" promoters by RNA polymerase found in the catalog.

Recognition of "extended-10" promoters by RNA polymerase

Tom Burr

Recognition of "extended-10" promoters by RNA polymerase

by Tom Burr

  • 179 Want to read
  • 34 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D) - University of Birmingham, School of Biosciences, Faculty of Science.

Statementby Tom Burr.
The Physical Object
Paginationxiii,151p. :
Number of Pages151
ID Numbers
Open LibraryOL18900247M

RNA Polymerase produces and releases short RNA transcripts of. Background6S RNA from E. coli is known to bind to RNA polymerase interfering with transcription initiation. Because 6S RNA concentrations are maximal at stationary phase and binding occurs preferentially to the holoenzyme associated with σ70 (Eσ70) it is believed that 6S RNA supports adjustment to stationary phase transcription. Previous studies have also suggested that .

Nucleoside-containing metabolites such as NAD + can be incorporated as 5′ caps on RNA by serving as non-canonical initiating nucleotides (NCINs) for transcription initiation by RNA polymerase (RNAP). Here, we report CapZyme-seq, a high-throughput-sequencing method that employs NCIN-decapping enzymes NudC and Rai1 to detect and quantify NCIN-capped by: Sigma factors are multi-domain subunits of bacterial RNA polymerase (RNAP) that play critical roles in transcription initiation, including the recognition and opening of promoters as well as the initial steps in RNA synthesis. This review focuses on the structure and function of the major sigma class that includes the housekeeping sigma factor (Group 1) that directs the bulk of Cited by:

  Control of transcription is crucial for correct gene expression and orderly development. For many years, bacteriophage T4 has provided a simple model system to investigate mechanisms that regulate this process. Development of T4 requires the transcription of early, middle and late RNAs. Because T4 does not encode its own RNA polymerase, it must Cited by: Structural characteristics and functions of RNA Polymerase (RNAP) Clamp. Region o n the β and β’ subunits. These residues are mobile domains that are part of the "crab claw" structure that swing open and closed, effectively changing the size of the active site channel. β (beta) flap. The β-flap covers the RNA exit channel through which the newly formed RNA strand is released.


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Recognition of "extended-10" promoters by RNA polymerase by Tom Burr Download PDF EPUB FB2

Barne K, Bown J, Busby S, Minchin S () Region of the RNA polymerase σ70 subunit is responsible for the recognition of the “extended - 10 motif” at Cited by: Barne KA, Bown JA, Busby SJW, Minchin SD. Region of the Escherichia coli RNA polymerase sigma70 subunit is responsible for the recognition of the “extended” motif at promoters.

EMBO J. ; – [PMC free article]Cited by: Three key promoter elements, i.e., −10, −35, and T−15G−14N, are recognized by the σ subunit of RNA polymerase.

Among them, promoters with the −10 element and either −35 or T−15G. Specific Recognition of the Promoter Element by the Free RNA Polymerase Subunit Article (PDF Available) in Journal of Biological Chemistry (30) August with 48 Reads.

RNA polymerase (RNAP) acts at a critical juncture to link the genomic potential of an organism to the expression of this potential as RNA, protein, and ultimately catalytic, structural, and behavioral properties.

Studies in Bacillus subtilis revealed the importance of the conserved TG in the promoter region, as subsequently found in theCited by: RNA POLYMERASE AND PROMOTERS. The initiating RNA polymerase holoenzyme (Eς 70) has a molecular weight of about × 10 5 and contains five subunits with the stoichiometry α 2 ββ′ς (see Fig.

Fig.2). 2).The ς subunit is bound relatively weakly to the rest of the enzyme (the core polymerase, E); ς is responsible for specific promoter recognition by RNA polymerase but Cited by:   Briefly, 50 n m RNA polymerase and promoters were incubated in K-glu (without dithiothreitol) for 15 min at 37 °C; KMnO 4 was added to a final concentration of 10 m m, and the reaction was stopped after 30 s by adding 2-mercaptoethanol to a final concentration of m m.

For the kinetic reactivity experiment, samples were taken after When in dsDNA form, the aptamers function as strong promoters for the T.

aquaticus RNA polymerase σ A holoenzyme. Recognition of the aptamer-based promoters depends on the downstream GGGA motif from the aptamers' common sequence, which is contacted by σ A region and directs transcription initiation even in the absence of the −35 Cited by: Transcription by T7 RNA Polymerase.- Pribnow Box Recognition and Melting by Escherichia coli RNA Polymerase.- Extended Promoters.- Promoter Selectivity Control of RNA Polymerase.- The Effect of High Pressure on Transcription and on the Structure and Activity of E.

coli RNA Polymerase.- Ribosomal RNA Promoter-RNA Polymerase Interactions and. Most activators of transcription initiation that affect scontaining RNA polymerase (RNAP) are sequence-specific DNA-binding proteins that bind to recognition sites located upstream of the core promoter.

The chapter explains the mechanism of action of one of the most thoroughly characterized activators of σdependent transcription in Escherichia coli, the cyclic AMP Cited by: 1.

A class of sigma 70 promoters that lack region but has extended element for extra contact is possible.(pg ) UP-element An additional DNA element that binds RNA polymerase that is found in some strong promoters that direct expression of ribosomal RNA molecules. DNA circular dichroism (CD) and FRET suggest that the promoter DNA in the closed complex is distorted.

Modeling suggests two different orientations of the recognition helix of the RNA polymerase in the closed complex. We propose that the RNA polymerase recognizes the sequence dependent conformation of the promoter DNA in the closed by: 5.

Promoters are the DNA sequences from which RNA polymerase initiates a RNA chain. Burton and Burton ()[] proposed a simple model for promoter evolution from LUCA (the last universal cellular common ancestor of bacteria, archaea and eukaryotes; ~ to billion years ago) (Figure 1).Remarkably, ancient promoters are posited to be surprisingly similar to the promoter.

The determination of when and how genes are “turned on and off” is a challenge in pos-genomic era. Differences between two species are closer to gene expression and regulation than to gene structures (Howard & Benson, ).The first and key step in gene expression is promoter recognition by RNA polymerase enzyme (RNAP).Cited by: 7.

The RNA polymerase with σ 54 recognizes a specific subset of promoters with highly conserved sequences and architecture, completely different from other promoters, and where transcription initiation is always dependent on additional transcriptional factors 9 (de Lorenzo, Chapter 10 Cited by: Recruitment of the general transcription factor TFIID to the promoter is a crucial step in the initiation of transcription by RNA polymerase II (Pol II) (Hampsey, ).

The TFIID complex comprises the TATA-binding protein (TBP) responsible for specific binding to the TATA element found in many Pol II promoters and a set of 13–14 TBP.

In E. coli, most of the standard σ 70 ‐type promoters are recognized by a primary sigma factor, σ 70, in the exponential stage, whereas alternative sigma factors direct RNA polymerase to specific promoters in response to environmental signals.

If a chloroplast contains a single primary sigma factor that is essential for chloroplast Cited by: Fundamental Aspects of T7 RNA Polymerase Structure and Mechanism --Transcription by T7 RNA Polymerase --Pribnow Box Recognition and Melting by Escherichia coli RNA Polymerase --Extended Promoters --Promoter Selectivity Control of RNA Polymerase --The Effect of High Pressure on Transcription and on the Structure and Activity of E.

coli RNA. @article{osti_, title = {Structure of a bacterial RNA polymerase holoenzyme open promoter complex}, author = {Bae, Brian and Feklistov, Andrey and Lass-Napiorkowska, Agnieszka and Landick, Robert and Darst, Seth A.}, abstractNote = {Initiation of transcription is a primary means for controlling gene expression.

In bacteria, the RNA polymerase (RNAP). Expression of the plastid rRNA operon (rrn) during development is highly regulated at the level of transcription.

The plastid rrn operon in most higher plants is transcribed by the plastid-encoded RNA polymerase (PEP), the multisubunit plastid RNA polymerase from PrrnP1, a σtype promoter with conserved −10 and −35 core promoter by:. Promoter Structure x RNA polymerase recognizes and binds to specific sequences from CHEM at Texas Tech UniversityAuthor: Redraider.Mechanisms of Transcription presents a unique perspective on the fundamental processes of transcription.

A collection of distinguished authors draws together the underlying mechanisms involved in the process of transcription. This includes .Regulation of gene expression is achieved by the presence of cis regulatory elements; these signatures are interspersed in the noncoding region and also situated in the coding region of the genome.

These elements orchestrate the gene expression process by regulating the different steps involved in the flow of genetic information. Transcription (DNA to RNA) and translation Author: Aditya Kumar, Manju Bansal.